CREB1 Antikörper (AA 5-24)

Produktnummer ABIN211132

Dieser Kaninchen Polyklonal Antikörper erkennt spezifisch CREB1 in WB und IP. Er zeigt eine Reaktivität gegenüber Human, Schwein, Hund, Pferd, Kaninchen, Affe und Fledermaus.

Kurzübersicht für CREB1 Antikörper (AA 5-24) (ABIN211132)

Target: CREB1 (cAMP Responsive Element Binding Protein 1 (CREB1))

Reaktivität: Human, Schwein, Hund, Pferd, Kaninchen, Affe, Fledermaus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CREB1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP)

Produktdetails anti-CREB1 Antikörper

Bindungsspezifität: AA 5-24

Spezifität: Recognizes human CREB at 43kD. Species cross-reactivity: Broad species cross-reactivity expected due to conservation of immunogenic sequence.

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Elephant, Panda, Dog, Bat, Horse, Rabbit, Pig (100%) Mouse, Hamster, Bovine, Opossum, Turkey, Chicken (95%) Rat, Xenopus (90%).

Aufreinigung: Protein A purified

Immunogen: KLH-conjugated, synthetic peptide (C-SGAENQQSGDAAVTEAENQQ) corresponding to amino acids 5-24 of human CREB. The immunizing sequence is identical in pig, shares 19 of 20 residues with mouse and bovine and 18 of 20 residues with rat. Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Elephant, Panda, Dog, Bat, Horse, Rabbit, Pig (100%), Mouse, Hamster, Bovine, Opossum, Turkey, Chicken (95%), Rat, Xenopus (90%).

Type of Immunogen: Synthetic peptide - KLH conjugated

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IP, WB

Usage: Suitable for use in Western Blot and Immunoprecipitation. Western Blot: 0.5-2 mg/mL detects CREB from a HeLa nuclear lysate. Representative blot from a previous lot: HeLa nuclear lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-CREB ( 0.5 μg/mL). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Positive Antigen Control: HeLa nuclear extract. Add 2.5 μL of 2- mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels. Immunoprecipitation: 5 mg (will immunoprecipitated CREB from 500 mg of HeLa nuclear extract).

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.1 % sodium azide, 30 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Details zu CREB1

Target: CREB1 (cAMP Responsive Element Binding Protein 1 (CREB1))

Andere Bezeichnung: CREB1 / CREB

Hintergrund: Name/Gene ID: CREB1
Family: Transcription factor

Synonyms: CREB1, CREB-1, Transactivator protein, CREB

Gen-ID: 1385

UniProt: P16220

Pathways: TLR Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Thyroid Hormone Synthesis, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Regulation of Cell Size, Toll-Like Receptors Cascades, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma, Positive Regulation of fat Cell Differentiation